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CAR T-cell manufacturing conditions are critical to differentiation, metabolic fitness, and cytotoxic function, enhancing therapy efficacy and production efficiency.
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
Poster presented at AAI 2025. Immune cell-mediated cytotoxicity of CD19 CAR-T cells against Raji suspension tumor cells was monitored and measured using real-time whole-well live cellimaging on the xCELLigence RTCA eSight system with its 5x...
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
In this study, we present a robust approach to monitor and analyze the bystander effect of Antibody-Drug Conjugates in real-time using live-cell imaging andimpedance measurements with the Agilent xCELLigence RTCA eSight system.
AACR 2025 poster: Chronic anti-CD3/CD28 stimulation during cell expansion generates exhausted T cells with a distinct metabolic and cytotoxic phenotype
This poster aims to demonstrate the capability of impedancebased xCELLigence RTCA assays for virus and antibody titer, as well as for identification and characterization of antiviral drugs.
The poster introduces a multiparametric workflow that allows for evaluating contractility, viability, and metabolic function using validated human iPSC-derived cardiomyocytes to better understand the mechanisms associated with drug-induced...
There is a need for functional potency assays that can be used to rapidly test the efficacy of different immunotherapy approaches, including but not limited tosmall molecules, biologics, cell therapies, or combinations of these agents
The eSight can be used to study cytotoxicity kinetics of lymphocytes invading a deregulated extracellular matrix present in solid tumors.
This quick reference guide provides detailed instructions on how to prepare, perform, and evaluate the impedance-based TCID50 protocol using the xCELLigence RTCA Software Pro Virology module to determine viral titer
we demonstrated consistent production of CAR T-cells using the functionally closed and automated cell manufacturing Lonza Cocoon platform, using a novelcombination of cell analysis parameters obtained from an Agilent xCELLigence RTCA system, a...
The invasion of the 3D spheroid in the presence or absence of drug treatment was monitored and quantified using the brightfield and fluorescence live cell time-lapse
Specification data sheet for the Agilent xCELLigence Real-Time Cell Analyzer HT-Biotek BioSpa 8.
Increased flexibility, complete data and more insight into cell health and behavior with the xCELLigence RTCA DP instrument.
Multiparametric real-time automated readouts using simultaneous cellular impedance and live cell imaging.
This editorial showcases Agilent solutions for metabolic modulation enhancing CAR T-cell activation, persistence, and serial killing for improved cancer immunotherapy
SPS 2024. Poster 112-D. To explore the potential on/off target immune-mediated toxicity of EpCAM-CD3 BiTE antibodies, a panel of human primary cells representing different tissues was exposed to EpCAM BiTE in the presence of PBMCs and monitored in...
CAR-TCR 2024 Poster. We demonstrated the successful production of CAR T-cells using the Lonza Cocoon system. Additionally, CAR T-cell in-process analyses and critical process parameters were evaluated using the combination of a flow cytometer, a...
In this application note, Antibody Analytics (Motherwell, UK) integrates IndEx-2, an advanced in vitro cell-based platform engineered to modulate the expression of one or two target antigens across a range of biologically relevant levels, with the...